The invention discloses a preparation method of surface amino-group functionalization SBA-15 mesoporous silica, and belongs to the technical field of organic functionalization ordered mesoporous materials synthetic method. The method comprises the following steps of taking aryl groups of different structures as protecting groups to react with silane coupling agents to prepare alkyl group trialkoxysilane functional precursor; under an acidic condition, taking tri-block non-ionic surface active agents as structure directing agents, and after pre hydrolyzing precursor, carrying out copolymerization with tetraethoxysilane or tetramethylsilane. The preparation method is characterized in that the aryl groups of different structures are taken as the protecting groups, so the functional precursor can be simply, conveniently and quickly prepared, and through the co-template role of the aryl groups and the structure directing agents, SBA-15 mesoporous silica, of which amino height is located at the inner surface of a duct, the microstructure is ordered and the bore diameters are different, is obtained.