The invention discloses a new method of measuring intracellular protein content standardization. The method is mainly achieved by means of preparation of standard curve and measurement of a to-be-test sample, wherein a DNA fluorescence staining method is employed. DNA is a genetic material in cells, so that for different cells in same individual body, the content of DNA is relatively stable. By means of the DNA for standardization to the to-be-test protein, various defects of protein standardization can be overcome.