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发明公开
CN104726441A 一种离子液体调控生物酶及其降解环境激素西维因的方法和降解率测定方法 失效 - 权利终止

一种离子液体调控生物酶及其降解环境激素西维因的方法和降解率测定方法
摘要：
本发明涉及一种离子液体调控生物酶及其降解环境激素西维因的方法和降解率测定方法，离子液体调控生物酶包括在pH值为2～5的磷酸缓冲液中加入氯过氧化物酶、季铵盐类离子液体和H2O2形成的混合溶液，在混合溶液中，氯过氧化物酶的浓度为0.005～0.05μmol·L-1，季铵盐类离子液体的浓度为0.06～0.20mol·L-1，H2O2的浓度为0.05～0.5mmol·L-1。本发明采用氯过氧化物酶为生物催化剂，季胺盐类离子液体为调控因子，季胺盐类离子液体具有对氯过氧化物酶的共溶剂作用，增加氯过氧化物酶的稳定性，增大了酶催化速率，提高氯过氧化物酶的有效利用率，应用于西维因的降解时，能够有效改善西维因的溶解性，提高降解率。
摘要（英）：

The invention relates to an ionic liquid regulation-control bio-enzyme, a method for degrading environmental hormone carbaryl by the same, and a method for determining the degradation rate. The ionic liquid regulation-control bio-enzyme comprises the mixed solution formed by adding chloroperoxidase, quaternary ammonium salt ionic liquids and H2O2 in a phosphate buffer solution with a pH value of 2-5, wherein in the mixed solution, the concentration of chloroperoxidase is 0.005-0.05mu mol.L<-1>, the concentration of ammonium salt ionic liquids is 0.06-0.20mol.L<-1>, and the concentration of H2O2 is 0.05-0.5mmol.L<-1>. According to the ionic liquid regulation-control bio-enzyme, the method for degrading environmental hormone carbaryl by the same, and the method for determining the degradation rate disclosed by the invention, the chloroperoxidase is used as a biocatalyst, the ammonium salt ionic liquids are used as regulation-control factors, the ammonium salt ionic liquids have a cosolvent effect on the chloroperoxidase, and can be used for improving the stability of the chloroperoxidase, increasing the enzyme catalysis speed and increasing the effective utilization rate of the chloroperoxidase; the ionic liquid regulation-control bio-enzyme is capable of effectively improving the solubility of carbaryl and increasing the degradation rate in case of being applied to degradation for carbaryl.

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