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发明授权
CN104678099B COX‑2在制备诊断、检测犬肿瘤的诊断试剂中的应用 失效 - 权利终止

COX‑2在制备诊断、检测犬肿瘤的诊断试剂中的应用
摘要：
本发明公开了COX‑2在制备诊断、检测犬肿瘤的诊断试剂中的应用：本发明通过实验研究发现，COX‑2在犬肿瘤(乳腺肿瘤，皮肤及软组织肿瘤，肛周肿瘤，睾丸肿瘤，阴道及子宫肿瘤)中的阳性表达率为76.1％，而在正常组织中的阳性表达率仅为20％，因此，COX‑2可以作为犬肿瘤的标志物进行应用，用免疫组织化学方法检测COX‑2的表达情况，其结果可以作为是否患有犬肿瘤的临床诊断的依据之一。本发明还公开了用于检测COX‑2的试剂盒(包括3％H2O2溶液、柠檬酸缓冲液、PBS缓冲液、小鼠COX‑2单克隆抗体、山羊抗小鼠IgG抗体、DAB显色液和蒸馏水)及检测方法(链霉素抗生物素蛋白‑过氧化酶染色)。
摘要（英）：

The invention discloses an application of COX-2 (cyclo-oxygen-ase-2) in preparation of a diagnostic reagent for dog tumor diagnosis and detection. The experiment research shows that the positive expression rate of COX-2 in dog tumors such as a breast tumor, skin and soft-tissue tumors, a perianal tumor, a testicular tumor and vagina and uterine tumors is 76.1%, the positive expression rate of COX-2 in normal tissue is only 20%, so that COX-2 can be applied as a dog tumor marker, the expression condition of COX-2 is detected with an immunohistochemical method, and a result can be taken as one of evidence items for clinically diagnosing whether a person suffers from the dog tumor. The invention further discloses a kit for detecting COX-2 and a detection method. The kit contains a 3% H2O2 solution, a citrate buffer solution, a PBS (poly butylenes succinate) buffer solution, a mouse COX-2 monoclonal antibody, a goat anti-mouse IgG antibody, a DAB (diaminobenzidine) developing solution and distilled water. The detection method comprises the step of streptaridin-peroxidase dyeing.

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