The invention relates to the field of an edible mushroom culture technology, specifically to an agrocybe cylindracea liquid strain culture solution and a culture method of an agrocybe cylindracea liquid strain. The agrocybe cylindracea liquid strain culture solution comprises the following ingredients: soya bean meal, white sugar, carrot meal, magnesium sulfate, dipotassium hydrogen phosphate, an antifoaming agent and water. The culture method of the agrocybe cylindracea liquid strain is to culture the agrocybe cylindracea liquid strain by the use of the above agrocybe cylindracea liquid strain culture solution. The culture method comprises the following steps: Step 1, culture of slant tube strain; Step 2, inoculation in a triangular flask; Step 3, culture of triangular flask strain; Step 4, inoculation in a fermentation tank; and Step 5, culture of fermentation tank strain. The agrocybe cylindracea liquid strain cultured by the use of the agrocybe cylindracea liquid strain culture solution has advantages of high vigour and strong vitality. Thus, dosage of the cultured agrocybe cylindracea liquid strain used for cultivation of agrocybe cylindracea is small. The culture method of the agrocybe cylindracea liquid strain has characteristics of simple method and short culture time.