The invention discloses a feed additive for laying hens, and application of the feed additive. The feed additive is prepared from the following raw materials by weight ratio: 10-20% of fucose, 5-10% of galactose, 5-10% of N-acetylgalactosamine, 10-20% of glucosamine, 10-15% of N-acetylglucosamine, 5-15% of glucuronic acid, 10-15% of mannose, 5-10% of sialic acid and 5-10% of xylose. The 0.001-5 weight percent of feed additive is fed into basic ration of the laying hens. The feed additive is rich in biological active substances, and is capable of promoting the growth of the laying hens, improving the disease resistance of the laying hens, effectively improving the feed utilization rate, increasing the laying rate of the laying hens, prolonging the peak laying period and improving the breeding efficiency; the eggs laid by the laying hens fed by the feed additive are delicious; the feed additive is free from toxic and side effects and drug residue, green and safe.