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发明授权
CN104541168B 基于免疫测定法的溶液中结合动力学的测定 有权

基于免疫测定法的溶液中结合动力学的测定
摘要：
本文报道了用于测定结合物与其配体的结合亲和力的方法，所述方法包含如下步骤:在样品中包含至少两个不同结合物:配体比率的结合物，配体和结合物‑配体‑复合物的样品中，基于免疫测定法结果测定游离结合物分数，并且如果测定的游离结合物分数不是对于所有使用的结合物：配体比率是可比的，则降低样品中结合物：配体比率，并通过相同的免疫测定法重新分析样品，并根据上述步骤中的游离结合物分数计算结合物与其配体的结合亲和力。
摘要（英）：

Herein is reported a method for the determination of the binding affinity of a binder and its ligand comprising the step of determining based on the result of an immunoassay the fraction of free binder in a sample comprising binder, ligand and binder-ligand-complexes for at least two different binder:ligand ratios in the sample, and if the determined fraction of free binder is not comparable for all used binder:ligand ratios then the binder:ligand ratio in the sample is lowered and the sample is re-analyzed by the same immunoassay, and calculating based on the fraction of free binder in the previous step the binding affinity for the binder to its ligand.

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