The aim of the present invention lies in providing superior dispersibility and retention of conductive particles in anisotropically conductive film and to maintain reliable conductivity even between terminals at a narrow pitch. A method for manufacturing anisotropically conductive film (1) containing conductive particles (3), wherein conductive particles (3) are embedded in the grooves (10) of a sheet (2) in which the grooves (10) have been formed continuously in the same direction, the conductive particles (3) are aligned, a first resin film (4) in which a thermocured resin layer (5) has been formed on top of stretchable base film (6) is laminated on the surface of the sheet (2) on the side in which the grooves (10) are formed, the conductive particles (3) are transferred, the first resin sheet (4) is uniaxially stretched in a direction other than the direction perpendicular to the direction in which the conductive particles (3) are aligned, and a second resin film (7) is laminated.