The invention provides a display panel, a manufacturing method of the display panel and a display device. The display panel comprises a first substrate, a second substrate, a liquid crystal layer located between the two substrates, a first orientation layer located between the first substrate and the liquid crystal layer and a second orientation layer located between the second substrate and the liquid crystal layer. A cushion object is arranged on the surface of the first substrate. A protrusion making contact with the cushion object is arranged in the position, corresponding to the cushion object, on the surface of the second substrate. Due to the fact that the protrusion opposite to the cushion object on the first substrate is arranged on the surface of the second substrate and the protrusion makes direct contact with the surface of the second substrate, the cushion object is prevented from making direct contact with the orientation layer on the second substrate, a liquid crystal panel can be protected, the orientation layers can not fall under the condition of vibration or impact, and liquid crystal is prevented from being polluted.