The invention discloses a preparation method of high-strength and high-toughness packing steel strip. The preparation method of the high-strength and high-toughness packing steel strip comprises the following steps: (1) selecting and preparing steel material components, wherein the selected steel material comprises the following components in percentage by weight: 0.3-0.37% of carbon, 0.05-0.08% of chromium, 0.45-0.75% of silicon, 1.53-1.75% of manganese, less than or equal to 0.19% of phosphorus, less than or equal to 0.065% of sulfur and the balance of iron; (2) smelting; (3) continuous casting; (4) descaling; (5) rolling; (6) coiling, cooling and storing the steel strip in a storehouse. The preparation method of the high-strength and high-toughness packing steel strip is simple in process and low in cost; the temperature and time for heating a steel slab are reasonably controlled and adjusted; the strength and toughness of the steel strip are effectively improved; the yield and quality of the steel strip are improved; the resource waste is effectively avoided; the prepared steel strip is stable in quality, good in property and wide in market prospect.