The invention discloses a gearshift manipulating mechanism overall height testing fixture comprising a bottom plate, a supporting plate and two positioning pins. The gearshift manipulating mechanism overall height testing fixture also comprises an overall height measuring plate and a centering plate which is used for fixing an operation rod in a neutral position. The overall height measuring plate is a step-type plate composed of a go segment and a no-go segment. In detection, a gearshift manipulating mechanism is placed on the bottom plate, and the two positioning pins are inserted into the installing holes of the supporting base body of the gearshift manipulating mechanism. Then the centering plate is inserted into a second positioning jack, the operation rod is limited in the neutral position via a U-shaped clamping groove, and then the overall height measuring plate is inserted into a first positioning jack. If the go segment of the overall height measuring plate does not interfere with the upper end of the operation rod and the no-go segment interferes with the operation rod, the overall height position of the gearshift manipulating mechanism is proved to be correct, or the position is proved to be wrong. The overall height of the gearshift manipulating mechanism is detected via the testing fixture so that operation is convenient and efficiency of detection work is high.