The invention discloses a detection kit for a camptodactyly pathogenic gene and application of the detection kit. The detection kit comprises a primer which is designed according to sequences of all exons and exon-intron boundary regions of a TLN2 gene, the primer is used for PCR amplification, PCR amplification products comprise nucleotide sequences of exons and exon-intron boundary regions of the TLN2 gene. The detection kit can be applied in the detection of the camptodactyly pathogenic gene; by virtue of the detection kit, whether the sequences of the human TLN2 gene encoding region are mutant or not is detected so as to determine that the TLN2 gene is the camptodactyly pathogenic gene; the detection is convenient and high in accuracy.