知搜-全球专利检索

  1. 登录
  2. 注册
发明授权
CN104328217B 一种测定嗜盐古生菌噬菌体SNJ1效价的引物及方法 失效 - 权利终止

一种测定嗜盐古生菌噬菌体SNJ1效价的引物及方法
摘要：
一种测定嗜盐古生菌噬菌体SNJ1效价的引物及方法，该引物包括上游引物5’-ACG AGT GGC GGC AGG TTT-3’、下游引物5’-TCC CGA TGA TGC GGT TGT-3’，利用上述引物测定嗜盐古生菌噬菌体SNJ1效价的方法时，先将待测噬菌体保存液与上游引物、下游引物、定量PCR试剂盒以及无菌水混合进行定量PCR反应，再于反应结束后测定Ct值，最后根据Ct值计算出嗜盐古生菌噬菌体SNJ1的效价。本发明不仅简便快速，而且特异性强、准确度高、重现性好。
摘要（英）：

The invention provides a primer and a method for measuring the titer of a haiophilic archaea phage SNJ1. The primer comprises an upstream primer 5'-ACGAGTGGCGGCAGGTTT-3' and a downstream primer 5'-TCCCGATGATGCGGTTGT-3'; according to the method for measuring the titer of the haiophilic archaea phage SNJ1 by use of the primer, firstly, a phage preserving fluid to be tested is mixed with the upstream primer, the downstream primer, a quantitative PCR kit and sterile water to have a quantitative PCR reaction; after the reaction ends, the Ct value is measured; and finally, the titer of the haiophilic archaea phage SNJ1 is calculated according to the Ct value. The primer and the method for measuring the titer of the haiophilic archaea phage SNJ1 are simple and fast, and strong in specificity, high in accuracy and good in reproducibility.

公开/授权文献
0/0