The invention belongs to the signal anti-interference technical filed and discloses a pulse anti-interference method and device. The pulse anti-interference method of the invention includes the step that n times of sampling detection is performed on pulse signal electrical levels with a set period, if n-times sampling values are continuous normal pulse signal electrical levels, it can be judged that normal pulses are detected, if other electric levels appear in the n-times sampling values, it can be judged that interference pulses exist, wherein the n is a positive integer and is large enough to ensure the reliability of the detection. The invention also discloses a pulse anti-interference device. According to the pulse anti-interference method and device of the invention, the pulse electrical levels are sampled, so that interference can be eliminated, and the anti-interference ability of equipment can be greatly improved, and as a result, the equipment can withstand 12kV electrostatic pulse interference. The pulse anti-interference method of the invention is flexible to operate, and sampling parameters (sampling frequency) can be adjusted according to an actual environment in which the equipment operates. With the pulse anti-interference method and device adopted, interference can be eliminated, and the use of hardware can be decreased, and the space of a circuit board can be saved, and product cost can be reduced.