The invention relates to a positioning device for a pre-embedded anchoring bolt. The positioning device comprises a vertical supporting assembly, a transverse limiting assembly and a positioning assembly, wherein the transverse limiting assembly is connected with the upper end part of the vertical supporting assembly in a moving manner; the positioning assembly is arranged at the free end of the transverse limiting assembly and is used for limiting the position of the anchoring bolt. According to the positioning device, the transverse limiting assembly is enabled to rotate by 360 degrees along the horizontal direction, the position of the positioning assembly can be conveniently adjusted, and the positioning assembly can be repeatedly used.