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CN104152540A 检测梅花鹿皮肤松弛症相关基因Fibulin-5的601C>G突变的试剂盒 失效 - 权利终止

检测梅花鹿皮肤松弛症相关基因Fibulin-5的601C>G突变的试剂盒
Abstract:
本发明涉及一种检测梅花鹿Fibulin-5基因突变的试剂盒，该试剂盒包括扩增Fibulin-5基因的试剂盒检测Fibulin-5基因突变的试剂。所属扩增Fibulin-5基因的试剂包括扩增Fibulin-5基因的PCR引物及相应的PCR反应试剂，其中所述扩增Fibulin-5基因的PCR引物是针对梅花鹿Fibulin-5基因第601位核苷酸，检测其是否存在C>G突变。所述PCR引物序列包括F382：5’-CTCTCTGTCTTCCAAGTCCCG-3’，R800：5’-ACAGGTGTAGCCTCCTTCGGT-3’。本发明研究发现，后颈部皮肤松弛梅花鹿Fibulin-5基因第601位核苷酸发生C>G突变。本发明所述引物、方法和试剂盒具有较高的扩增稳定性、特异性、灵敏度高、准确性高等优点。
Abstract(English):

The invention relates to a kit for detecting the 601C>G mutation of sika-deer cutis laxa related genes Fibulin-5. The kit comprises a reagent for detecting the mutation of Fibulin-5 genes in a kit for amplifying Fibulin-5 genes. An affiliated reagent for amplifying Fibulin-5 genes comprises a PCR primer for amplifying Fibulin-5 genes and a corresponding PCR reaction reagent, wherein the PCR primer for amplifying Fibulin-5 genes is designed for detecting whether the 601st nucleotide of a sika-deer Fibulin-5 gene has C> mutation. The sequences of the PCR primer include F382:5'-CTCTCTGTCTTCCAAGTCCCG-3', and R800:5'-ACAGGTGTAGCCTCCTTCGGT-3'. According to the invention, researches show that the 601st nucleotide of a sika-deer back-neck cutis laxa related Fibulin-5 gene has C> mutation. The primer, method and kit disclosed by the invention have the advantages of high amplification stability, high specificity and sensitivity, high accuracy and the like.

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