The invention discloses a screen positioning method. The screen positioning method comprises the steps that when it is detected that a laser light source emits laser light onto a display screen, the display screen is controlled to display a preset image within a preset period of time, and an acquisition device is controlled to acquire a light spot image formed when the laser light is emitted onto the preset image which is displayed at present; the light spot image is obtained from the acquisition device, and a sample image which is matched with the light spot image is searched for in the prestored sample images; location information relevant to the sample image matched with the light spot image is obtained, and then the position where the laser light shines on the display screen is obtained. Compared with the prior art, the screen positioning method has the advantages of being simple, easy to realize and more accurate.