The invention discloses a method for cultivation of Yellow Sea gadus macrocephalus parent plants and incubation of oosperms, and belongs to the technical field of aquiculture seed cultivation. The method comprises the steps of parent plant cultivation and ovum pick-up, and artificial insemination and incubation; the parent plant cultivation temperature is at 2-8 DEG C; temperature fluctuation stimulation for one time is conducted during the parent plant cultivation period; the temperature stimulation method is that the water temperature uniformly and progressively rises to 6-8 DEG C from 2-3 DEG C; the temperature rising period lasts for 7-9 days; the temperature of 6-8 DEG C lasts for about 4-5 days; then the water temperature progressively and uniformly reduces to 3-4 DEG C from 6-8 DEG C; temperature reduction period lasts for 6-8 days; then the water temperature maintains at 3-4 DEG C; illumination intensity is adjusted to 10001 lx-15001 lx; salinity is at 2.8-3.3%; pH value is at 7.0-8.5; the cultivation is conducted in running water. According to the invention, the temperature stimulation is conducted during the parent plant cultivation process, so as to conducive to sex gland development of the parent plants; incubation is conducted on oosperms adopting the method; the hatchability is about 41.2%.