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发明公开
CN104059898A 一种高效表达V8蛋白酶的方法 无效 - 驳回

一种高效表达V8蛋白酶的方法
摘要：
本发明公开了一种高效表达V8蛋白酶的方法，属于酶工程技术领域。本发明将融合了SUMO标签的V8蛋白酶基因，在大肠杆菌中进行表达，获得含有SUMO标签的V8蛋白酶，然后利用SUMO蛋白酶移除SUMO标签，获得具有活性的V8蛋白酶。酶活可达15.5U/mg，已经适用于重组蛋白制备和肽指纹图谱鉴定。
摘要（英）：

The invention discloses a method for efficiently expressing V8 proteinase, belonging to the technical field of enzyme engineering. The method comprises the following steps: expressing a V8 proteinase gene fused with an SUMO label in Escherichia coli, thus obtaining V8 proteinase containing the SUMO label; and removing the SUMO label by using SUMO proteinase, thus obtaining active V8 proteinase. The enzyme activity can be up to 15.5U/mg. The method has been used for recombinant protein preparation and peptide fingerprint identification.

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