The present invention relates to a stable aqueous suspension herbicide composition containing glyphosate, and a preparation method thereof. The herbicide composition of the present invention comprises: a glyphosate salt and saflufenacil as herbicidal active ingredients; and one or more types of surfactants selected from the group consisting of (a) a polyoxyalkylene alkyl ether, (b) polyoxyethylene sorbitan fatty acid ester, and (c) a polyoxyalkylene alkyl amine, an alkyl polyglycoside, and a tetraalkyl ammonium organic or inorganic salt, as an effect enhancer.