A method for implementation of access control on an electric power machine room based on a two-dimensional code and a radio frequency identification (RFID) technology comprises a system composed of a mobile phone, a two-dimensional code scanner, an RFID card reader, an RFID embedded tag, an access control device, an access control digital information recognition program and having functions of two-dimensional code image recognition, user identity radio frequency identification automatic inspection and access control management; when an electric power machine room worker needs to enter the machine room to work, the two-dimensional code or the embedded RFID tag in the mobile phone can be used for identity verification, the system verifies worker information according to an actual verification mode and controls opening and closing states of the access control according to a verification result. Convenient and effective automation management of the access control on the electric power machine room is carried out based on the two-dimensional code and the RFID technology, and the method has the remarkable advantages of simple and easy use, reliability and wide use range.