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CN103958670A 附着软骨细胞的多孔质体的长期保存方法 无效 - 驳回 复审申请

附着软骨细胞的多孔质体的长期保存方法
摘要：
本发明揭示了一种在密封状态下至少在14天内长期保存附着软骨细胞的多孔质体的方法。该方法具备以下部分：将分离了的软骨细胞以1.0×107个细胞/ml～1.0×108个细胞/ml的给与细胞浓度悬浊于去端肽胶原液中制备细胞悬浊液，将上述细胞悬浊液接种在具备生物体适宜性的多孔质体上，使上述细胞悬浊液在上述多孔质体中凝胶化得到附着软骨细胞的多孔质体，将上述附着软骨细胞的多孔质体在给与活细胞数量每1mL为1.0×105个细胞以上的量的含有胰岛素、成纤维细胞生长因子以及5％以上的血清的培养基中，以培养基的振荡速度在大于0cm/秒且在2.8cm/秒以下的范围内的方式进行振荡的同时，维持26～37℃。
摘要（英）：

Disclosed is a method for long-term storage of a chondrocyte-bearing porous body in a sealed state for at least 14 days. The method comprises: suspending isolated chrondrocytes in an atelocollagen solution at an administered cell concentration of 1.0*107-1.0*108 cells/mL to prepare a cell suspension; seeding a biocompatible porous body with the cell suspension; gelling the cell suspension within the porous body to obtain a chrondrocyte-bearing porous body; and maintaining the chrondrocyte-bearing porous body at 26-37 DEG C in a culture comprising insulin, fibroblast growth factor, and 5% or more serum, in the amount of at least 1 mL per 1.0*105 administered cells, with shaking at a culture shaking speed within a range greater than 0 cm/s to 2.8 cm/s.

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