The invention discloses a tool kit for manually detaching and assembling a dual-layer damping fastener for an urban rail. The tool kit comprises a locking ring detaching tool and a fastener body assembling and detaching tool. The locking ring detaching tool comprises four claws, a plurality of hinge pins, a fixing support, an adjusting nut, a sliding rod, a sliding ring, two handles and a connecting disc. The fastener body assembling and detaching tool comprises a positioning bottom plate, a pressing support, a sliding block, a rotating wheel, a crank, an idler wheel shaft, a cam and a cam shaft. Compared with the prior art, the tool kit has the advantages that the tool kit is provided with simple tools, is easy to carry, allows a locking ring in the dual-layer damping fastener to be rapidly taken out, and can rapidly promote a lower layer iron cushion plate and a middle rubber cushion plate in a fastener body to move leftward so as to promote the fastener body to be separated, application cost of the fastener is reduced, and use economical efficiency of the damping fastener is improved.