The invention discloses an automatic immunodetection micro-fluidic chip fluorescent quantification detection device which comprises an excitation light source, an exciting light focusing module, a fluorescence collection module, a photoelectric conversion module, a linear motion platform module, a circuit module, an automatic reaction execution module and a waste reaction liquid removal module. In the detection process, a chip is subjected to one-dimensional quantitative scanning detection through linear light spots obtained by the exciting light focusing module; a controller obtains the concentration or cell number of a sample to be detected based on fluorescence signal intensity distribution information in a chip two-dimensional rule detection area; the detection result is connected with a mobile phone through Bluetooth and is transmitted to medical institutions in real time, and remote diagnosis or epidemic disease distribution area monitoring is realized. A linear light spot one-dimensional scanning detection method is adopted, and quantification of micro-fluidic chip detection is realized. Moreover, by means of the automatic reaction execution module, automation of the micro-fluidic chip detection is realized; due to the integrated waste reaction liquid removal module, the allowed reaction sample amount is increased, and the detection sensitivity is improved.