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发明公开
CN103695364A 通过弱化5-氨基乙酰丙酸脱水酶活性获得5-氨基乙酰丙酸高产菌株及其应用 有权

通过弱化5-氨基乙酰丙酸脱水酶活性获得5-氨基乙酰丙酸高产菌株及其应用
摘要：
本发明公开了一种构建5-氨基乙酰丙酸(ALA)生产菌株的方法，即弱化所述ALA生产菌株中5-氨基乙酰丙酸脱水酶的活性。本发明还公开了利用所述方法构建的ALA生产菌株和利用所述菌株制备ALA的方法。利用本发明的菌株无需添加外源5-氨基乙酰丙酸脱水酶抑制剂即可高产量、低成本产生ALA及其下游产物。本发明的方法还可与现有技术中其它构建ALA生产菌株的方法联用，从而进一步提高ALA的产量。
摘要（英）：

The invention discloses a method for building a 5-aminolevulinic acid (ALA) producing strain, namely by weakening the activity of 5-aminolevulinic acid dehydratase in the ALA producing strain. The invention also discloses the ALA producing strain built by using the method and the method for preparing ALA by using the strain. The ALA and downstream products thereof can be produced by using the strain disclosed by the invention at high yield and low production cost under the condition of not adding an exogenous 5-aminolevulinic acid dehydratase inhibitor. The method disclosed by the invention also can be combined with the other methods for building the ALA producing strain in the prior art. Thus, the yield of the ALA is further increased.

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