The invention relates to a technology for measuring and controlling flexible conductors in transformer substation projects, in particular to a three-dimensional simulation, measurement and control system for transformer substation project flexible conductor assembling. The three-dimensional simulation, measurement and control system is a prism-free, total-station-instrument and three-dimensional analog simulation model system and aims at finishing flexible conductor coordinate measurement and computer simulation of the overall three-dimensional space effect and then efficiently and safely finishing on-site conductor installation. An adopted total-station instrument performs contact type measurement, carries on research and verification on a mathematic model of the blanking length of a transformer substation project flexible conductor and designs a conductor three-dimensional space arrangement installation effect picture through a computer. The three-dimensional simulation, measurement and control system has the advantages that the system are used for installation of transformer substation overhead conductors and downleads, and the construction efficiency is improved; the power outage time is shortened and high-altitude construction operation risk is reduced in reconstruction and extension projects; the conductor installing accuracy is improved, and the construction appearance quality is high; a man-machine conversation interface is good, and operation is simple and convenient; the system is good in universality and suitable for various specifications of flexible conductor made of various materials.