Disclosed are a conductive alignment layer, a manufacture method of the conductive alignment layer, a display substrate comprising the conductive alignment layer and a display device which relate to the technology of LCD, simplify the manufacture method of conductive layer and alignment layer and also reduce the complexity in manufacturing a LCD device by preparing the conductive layer and the alignment layer in the substrate simultaneously through utilizing a material possessing both conductivity and alignment, without the need of preparing the conductive layer and the alignment layer separately.