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CN103667533A 一种人呼吸道博卡病毒半巢式PCR检测试剂盒及其非诊断性检测方法 失效 - 权利终止

  • Patent Title: 一种人呼吸道博卡病毒半巢式PCR检测试剂盒及其非诊断性检测方法
  • Patent Title (English): Semi-nested PCR detection kit and non-diagnostic detection method for human respiratory-tract bocavirus
  • Application No.: CN201310657249.5
    Application Date: 2013-12-10
  • Publication No.: CN103667533A
    Publication Date: 2014-03-26
  • Inventor: 孙玉宁 , 姚青 , 李建宁 , 张茜 , 杨怡 , 高玉婧
  • Applicant: 宁夏医科大学
  • Applicant Address: 宁夏回族自治区银川市胜利街1160号
  • Assignee: 宁夏医科大学
  • Current Assignee: 宁夏医科大学
  • Current Assignee Address: 宁夏回族自治区银川市胜利街1160号
  • Main IPC: C12Q1/70
  • IPC: C12Q1/70 ;  C12Q1/68 ;  C12R1/93
一种人呼吸道博卡病毒半巢式PCR检测试剂盒及其非诊断性检测方法
Abstract:
本发明公开了一种人呼吸道博卡病毒半巢式PCR检测试剂盒及其非诊断性检测方法。该试剂盒包括：1)病毒保护液，其成份为青霉素200U/ml、链霉素200U/ml、两性霉素B200U/ml和BSA0.125％；2)病毒裂解液，其成份为10mmol Tris-HCl、1mmol EDTA、15mmol NaCl和0.5％SDS，pH为8.0，溶剂为水；3)扩增引物，扩增引物为NS1545F、NS1962R和NS1835R。本发明对人呼吸道博卡病毒不仅检出率高，而且不用提取病毒基因组DNA，同时也不需应用荧光定量PCR技术进行检测，降低了临床患者博卡病毒的检测成本，提高了检测效率。
Abstract(English):

The invention discloses a semi-nested PCR detection kit and a non-diagnostic detection method for human respiratory-tract bocavirus. The kit comprises: 1) a virus protective solution comprising 200 U/mL of penicillin, 200 U/mL of streptomycin, 200 U/mL of amphotericin and 0.125% of BSA; 2) a virus lysate comprising 10 mmol of Tris-HCl, 1 mmol of EDTA, 15 mmol of NaCl and 0.5% of SDS, wherein the pH is 8.0 and the solvent is water; and 3) amplimers NS1545F, NS1962R and NS1835R. According to the kit and the method, the detection rate of human respiratory-tract bocavirus is high, viral genome DNA does not need extracting, and also the fluorescent quantitative PCR technology does not need applying for detection, so that the detection cost of bocavirus of clinical patients is reduced and the detection efficiency is improved.

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