The invention discloses an electrostatic interaction induced micelle preparation method, and belongs to the technical fields of natural macromolecules and macromolecule self-assembling. The method comprises the following steps: processing raw materials comprising chitosan and negatively charged polyanion or micro-molecules in an aqueous solution to form a cation-anion composite system, forming a cation chitosan based composite micelle in the stirring and mixing process, changing the ratio of anions to cations, and self-assembling in the aqueous solution to form the micelle having a particle size in a range of 200-600nm. The micelle preparation process is simple and environmentally-friendly, and has a short period. The micelle has a good stability, a good hydrophilicity, a good biocompatibility and a good emulsibility, and can be applied to the fields of cosmetics, foods, biomaterials, hydrophilic colloids, emulsions and the like.