The invention discloses a method for measuring content of formononetin in a Zhenqifuzheng preparation. The method is a high-performance liquid chromatography with a formononetin reference substance used as a contrast and the ratio of methyl alcohol to water/a 0.01%-5% phosphoric acid aqueous solution/a 0.01%-5% glacial acetic acid/a 0.01%-0.5% formic acid aqueous solution of (50-70):(50-30) or the ratio of acetonitrile to the water/the 0.01%-5% phosphoric acid aqueous solution/the 0.01%-5% glacial acetic acid/the 0.01%-0.5% formic acid aqueous solution of (20-50):(80-50) used as a mobile phase. The method is high in specificity, high in accuracy, good in repeatability, high in recovery rate, high in stability, accurate in measurement result and capable of achieving the purpose of effective control of the drug quality and ensuring the stability of the product quality and the safety and the effectiveness of clinical medication.