The invention discloses a sealing structure of a needle-shaped stop valve. The sealing structure comprises a valve rod, a valve core and a valve body, the valve core is arranged in the valve body, one end of the valve rod is connected with the valve core, a first sealing tape and a second sealing tape are arranged between the valve core and the valve body, the second sealing tape is arranged above the first sealing tape, and the first sealing tape and the second sealing tape are arranged on the valve body and realize sealing functions one after another. The sealing structure is provided with the two sealing tapes which can take part in sealing one after another when the needle-shaped stop valve works, and quick sealing failure caused by abrasion and wear of the valve core and a valve seat due to the fact that a handle is screwed excessively is reduced, so that service life of the needle-shaped stop valve is prolonged.