The invention provides a binding method of a network card interface. The binding method comprises the following steps of matching currently-acquired information of all network cards in a system with all entries stored in an MAC (Media Access Control) address record table in the system, and setting in-place identifiers of the interfaces of the matched entries to be in an in-place state. The binding method provided by the invention has the advantages that not only can the binding be carried out on network equipment developed by a virtual platform, but also the efficiency and correction rate of binding of the network card interfaces are increased. The invention also discloses a binding device of the network card interface.