The invention belongs to the technical fields of feeds and microbe fermentation, and particularly relates to a feed rich in conjugated linoleic acid and a preparation method of the feed. The preparation method comprises the following steps: carrying out size mixing on perila seed powder, maize meal, treacle and sunflower seed oil to prepare fermentation liquor; increasing the temperature, soaking, sterilizing, and then adding lactobacillus plantarum; carrying out primary liquid fermentation to prepare a conjugated linoleic acid liquid fermented feed, and then mixing grain bean pulp feeds; carrying out secondary solid-state fermentation, and then drying at a low temperature. The conjugated linoleic acid fermented feed disclosed by the invention is rich in the conjugated linoleic acid, small peptides, lactic acid and lactic acid bacteria, has the characteristics of enrichment and balance in amino acid, high nutrition, easiness for digestion, good palatability, low anti-nutritional factor content, and the like and can obviously enhance the lean meat rate of livestock and poultry, increase the feed intake of livestock and poultry and outstandingly promote the growth of the livestock and the poultry.