Provided is an information processing apparatus, including a testing section performing statistical testing on simple staining data obtained by performing fluorescence measurement on a particle subjected to simple staining with a staining material having a prescribed fluorescence characteristic and unstaining data obtained by performing fluorescence measurement on an unstained particle for comparison for a frequency band, a masking processing section setting, in a case where there is no significant difference between the simple staining data and the unstaining data for the frequency band, the simple staining data to 0 or a prescribed value, and an estimation section estimating, in a manner that double staining data obtained by performing fluorescence measurement on a particle stained with a plurality of staining materials is represented by a linear combination of base vectors representing a distribution of the simple staining data corresponding to each staining material, a combination coefficient of the linear combination.