The invention discloses a non-tempering biological particle feeding box and a working method. The feeding box comprises a first feeder and a second feeder which is arranged at a bottom outlet end of the first feeder, wherein the first feeder comprises a storage box, a vertical screw and a vertical motor; the bottom of the storage box is provided with a discharge port; the vertical screw is vertically arranged in the storage box; the vertical motor is arranged at the top end of the vertical screw for driving the vertical screw to rotate; the second feeder comprises a feeding pipe, a horizontal screw and a horizontal motor; the side wall of the feeding pipe is provided with a feed port and the feed port is butted against the discharge port of the storage box; the horizontal screw is rotatably arranged in the feeding pipe; the horizontal motor is connected with the horizontal screw and drives the horizontal screw to rotate. According to the non-tempering biological particle feeding box, fire can be effectively prevented from reaching a bin and fire burn-back is avoided.