The invention discloses a glue mixing and dispensing gun. The glue mixing and dispensing gun comprises a glue dispensing head, wherein a mixing cavity is connected above the glue dispensing head, an A glue cavity and a B glue cavity are vertically connected to the mixing cavity, and a temperature control ring is sleeved outside the mixing cavity. Compared with an original mode that two types of glue are firstly mixed through a three-way pipe and then delivered into a glue dispensing gun, an original cavity is divided into the A glue cavity and the B glue cavity, glue is pumped out by a pump and then directly flows into the glue dispensing gun to be mixed, so that glue mixing time is greatly shortened, the situation that the glue solidifies and accordingly causes blockage before being injected is avoided. In addition, the temperature control ring is sleeved outside the mixing cavity, so that the temperature can be monitored in real time and can be timely adjusted in a low-temperature environment, and glue solidification is prevented.