知搜-全球专利检索

  1. 登录
  2. 注册
发明公开
CN103276070A 鉴别花脸香蘑原生质体单核体交配型的方法及其专用引物对IS-879b 失效 - 权利终止

鉴别花脸香蘑原生质体单核体交配型的方法及其专用引物对IS-879b
摘要：
本发明公开了鉴别花脸香蘑原生质体单核体交配型的方法及其专用引物对IS-879b。该方法包括：分别以待鉴别的两株花脸香蘑原生质体单核体的基因组DNA为模板，用由SEQ ID No.1和SEQ ID No.2所示的两条单链DNA组成的PCR引物对进行PCR扩增，检测所得到的PCR产物的大小，如果所述待鉴别的两株花脸香蘑原生质体单核体的PCR产物均含有或均不含有1000bp-1500bp的DNA片段，所述待鉴别的两株花脸香蘑原生质体单核体的交配型相同；如果其中的一株含有1000bp-1500bp的DNA片段，另一株不含有1000bp-1500bp的DNA片段，所述待鉴别的两株花脸香蘑原生质体单核体的交配型不同。
摘要（英）：

The invention discloses a method for identifying the mating types of lepista sordida protoplasted monokaryons and a special primer pair IS-879b thereof. The method comprises the following steps of: taking genomes DNA of two lepista sordida protoplasted monokaryons to be identified as templates respectively, performing polymerase chain reaction (PCR) amplification by using a PCR primer pair consisting of two single chains DNA shown as SEQ ID No. 1 and SEQ ID No. 2, and detecting the magnitude of the obtained PCR products, wherein if the PCR products of both the two lepista sordida protoplasted monokaryons to be identified contain or do not contain DNA fragments of 1000bp-1500bp, the mating types of the two lepista sordida protoplasted monokaryons to be identified are identical; and if the PCR product of one protoplasted monokaryon contains the DNA fragments of 1000bp-1500bp and the PCR product of the other one does not contain the DNA fragments of 1000bp-1500bp, the mating types of the two lepista sordida protoplasted monokaryons to be identified are different.

公开/授权文献
0/0