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发明公开
CN103255108A 细胞粘附分子5单克隆抗体、制备方法及应用 有权 转让

细胞粘附分子5单克隆抗体、制备方法及应用
摘要：
本发明公开了细胞粘附分子5（ICAM5）单克隆抗体、制备方法及应用，其杂交瘤细胞株保藏编号为CGMCC No.5798。本发明提供ICAM5单克隆抗体，可用于针对ICAM5抗原的免疫荧光和蛋白印迹（Western blotting）等科学研究，经试验验证在人及小鼠神经细胞表面有很高的表达并且表达稳定。抗体特异性强，并且效价高，ELISA检测效价可达1：400000，因此试验中ICAM5抗体使用浓度较低，抗体用量少。ICAM5抗原位于神经细胞胞体及神经纤维表面，因此免疫荧光染色可清晰展示神经细胞胞体及神经纤维结构。
摘要（英）：

The present invention discloses cell adhesion molecule 5 (ICAM5) monoclonal antibody, a preparation method and applications thereof, wherein a preservation number of the hybridoma cell strain is CGMCC No.5798. The present invention provides the ICAM5 monoclonal antibody, which can be provided for ICAM5 antigen-based immunofluorescence and Western blotting, and other scientific researches, wherein test results show that the high and stable expression exists on the surface of human and mouse nerve cells. In addition, the antibody has characteristics of strong specificity and high titer, wherein an ELISA detection titer can be 1:400000, such that an ICAM5 antibody use concentration in a test is low, and an antibody use amount is low; and the ICAM5 antigen is positioned on the surface of nerve cell bodies and nerve fibers, such that structures of nerve cell bodies and nerve fibers can be clearly displayed with immunofluorescence staining.

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