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发明公开
CN103243071A 一种临床级人间充质干细胞无血清完全培养基 有权 质押 转让

  • 专利标题： 一种临床级人间充质干细胞无血清完全培养基
  • 专利标题（英）： Clinical-grade human mesenchymal stem cell serum-free complete medium
  • 申请号： CN201310180222.1
    申请日： 2013-05-09
  • 公开(公告)号： CN103243071A
    公开(公告)日： 2013-08-14
  • 发明人: 孙洪喜 , 张高峰 , 于丽 , 范友金 , 陈云燕
  • 申请人： 陈云燕
  • 申请人地址： 山东省青岛市城阳区兴阳路417号28号楼3单元601户
  • 专利权人： 陈云燕
  • 当前专利权人： 青岛瑞思德生物科技有限公司
  • 当前专利权人地址： 山东省青岛市城阳区兴阳路417号28号楼3单元601户
  • 主分类号： C12N5/0775
  • IPC分类号： C12N5/0775
一种临床级人间充质干细胞无血清完全培养基
摘要：
本发明涉及一种人间充质干细胞培养基。本发明的培养基，在基础培养基中添加有以下组分，各组分终浓度为：人血清白蛋白1～2g/L、转铁蛋白5～10mg/L、纤粘连蛋白2～8mg/L、层粘连蛋白1～4mg/L、Fe(NO3)3·9H2O50g/L、FeSO4·7H2O417g/L、雌二醇1～3μg/L、睾酮2～5μg/L、孕酮1～3μg/L、地塞米松39.25～117.74μg/L、胰岛素5～10mg/L、核黄素376.36mg/L、辅酶A80.96～242.87mg/L、丁二胺4.41～6.17mg/L、牛磺酸1～2mg/L、氨基乙醇0.61～1.85mg/L、丙酮酸8.81～26.42mg/L、硒酸钠3.78～7.56μg/L、L-谷氨酰胺292.3～584.6mg/L、血管内皮生长因子2～8μg/L、表皮生长因子4～10μg/L、碱性成纤维细胞生长因子4～10μg/L、白血病抑制因子1～5μg/L、胰岛素样生长因子-I1～5μg/L、干细胞因子2～8μg/L组成。本发明的培养基，不含动物血清，排除了动物血清内潜在动物源性内毒素或病毒，方便应用于临床。
摘要（英）：

The invention relates to a human mesenchymal stem cell culture medium. According to the culture medium, the basal culture medium comprises the following components based on the final concentration: 1-2g/L of human serum albumin, 5-10mg/L of transferring, 2-8mg/L of fibronectin, 1-4mg/L of laminin, 50g/L of Fe(NO3)3.9H2O, 417g/L of FeSO4.7H2O, 1-3mu g/L of estradiol, 2-5mu g/L of testosterone, 1-3mu g/L of progesterone, 39.25-117.74 mu g/L of dexamethasone, 5-10mg/L of insulin, 376.36mg/L of riboflavin, 80.96-242.87mg/L of coenzyme A, 4.41-6.17mg/L of butanediamine, 1-2mg/L of taurine, 0.61-1.85mg/L of aminoethanol, 8.81-26.42mg/L of pyruvic acid, 3.78-7.56mu g/L of sodium selenate, 292.3-584.6mg/L of L-glutamine, 2-8mu g/L of vascular endothelial growth factor, 4-10mu g/L of epidermal growth factor, 4-10mu g/L of basic fibroblast growth factor, 1-5mu g/L of leukaemia inhibitory factor, 1-5mu g/L of insulin-like growth factor-I and 2-8mu g/L of stem cell factor. The culture medium does not contain the animal serum, the potential animal endogenous endotoxin or virus of the animal serum is eliminated, and the culture medium is conveniently applied to clinics.

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