The invention discloses a new use of a drug of a chain amide CCR5 (chemokine receptor) receptor antagonist. The new use provided by the method is the chain amide CCR5 inhibitor or an application of pharmaceutically acceptable salt in the preparation of a spermicidal drug. The structure formula of the chain amide CCR5 inhibitor is shown in formula I. An inactivating sperm in vitro test proves that the series of compounds have strong sperm killing activity; the EC50 value of the compound with the strongest activity achieves 1.5mg/ml; and the chain amide CCR5 inhibitor can develop into a preparation for in vitro contraception, or a multifunctional external preparation for resisting aids and contraception.