The invention discloses an aquatic animal temperature selection experimental system. The aquatic animal temperature selection experimental system is characterized by comprising an outer water tank (1), wherein an inner water tank (2) which is made of heat conduction materials is arranged along the axial direction of the outer water tank (1) and in the outer water tank (1), the inner water tank (2) and the outer water tank (1) are not communicated with each other, an inlet end of the inner water tank (2) is communicated with a heating water tank (3) through a pipeline, a heater (4) is arranged inside the heating water tank (3), an outlet end of the inner water tank (2) is connected with a recovery water tank (5) through a pipeline, and the outer water tank (1) is connected with a cooling-water machine (6) through a water inlet and outlet pipe. The aquatic animal temperature selection experimental system is simple in structure, exquisite in design, capable of providing continuous water temperature changes, and capable of carrying out research on water temperature application ranges of aquatic animal adults and larvae such as fishes, shrimps, crabs, cephalopodas and the like from the ethology view.