The invention relates to a micro-fluidic chip, a hemocyte analysis system and a hemocyte analysis method. The micro-fluidic chip comprises at least one detection flow channel, wherein each detection flow channel comprises a first sample inlet, a second sample inlet, a sample micro flow channel, an auxiliary solution micro flow channel and a convergence micro flow channel, wherein the sample micro flow channel is connected with the first sample inlet; the auxiliary solution micro flow channel is connected with the second sample inlet; the convergence micro flow channel is connected with the sample micro flow channel and the auxiliary solution micro flow channel; a small hole is formed in the convergence micro flow channel; and a first electrode and a second electrode are respectively arranged on two sides of the small hole. Hemocytes can be accurately counted by measuring sizes of hemocytes of a sample to be detected by detecting voltage change between the first electrode and the second electrode of the micro-fluidic chip, so that the measuring cost is greatly reduced, and the measuring efficiency is improved.