The present invention relates to a composite flora for biological preparation of textile fibers, and applications thereof. The composite flora comprises bacillus sp. with a preservation number of CGMCC No.5971, acinetobacter lwoffii with a preservation number of CGMCC No.5973, and pseudomonas fluorescens with a preservation number of CGMCC No.5974. The method comprises the following steps: preparing a bacterial liquid; treating raw materials; and preparing fibers, wherein fiber preparation comprises biodegradation, steam sterilization, fiber obtaining, drying, and combing. According to the present invention, the method does not pollute the environment, a waste liquid is directly converted into an organic fertilizer so as to achieve zero emission and zero pollution, the biological treatment process provides a protection effect for fibers, advantages of low production cost and high economic benefits are provided compared with the traditional chemical method, and characteristics of energy source saving and environmental protection are provided.