The invention relates to a fabrication method of a surface-enhanced Raman probe based on secondary enhancement. The surface-enhanced Raman probe consists of a pyramid-like micro-tip structure coated with a metal film and metal nanoparticles on the tip. The micro-tip structure excites and propagates surface plasma waves along the metal film surface, and the surface plasma waves gather at the tip to form a primary local enhancement field, which serves as an exciting field to excite the metal nanoparticles to generate a further local to form a secondary local enhancement field stronger than the primary local enhancement field, so that a higher enhancement factor can be obtained to enable the probe to realize high-sensitivity surface-enhanced Raman detection. The surface-enhanced Raman probe fabricated by the method provided by the invention solves the problems of the conventional Raman detector, such as low enhancement factor, and can realize high-sensitivity surface-enhanced Raman detection.