The invention relates to a kit for detecting vasculitis related autoantibody repertoire, which comprises a film strip, ELISA (Enzyme Linked Immunosorbent Assay) solution, a substrate and concentrated washing and incubation solution. The film strip consists of a piece of slide glass, and antigen strips, cut-off control strips and a function control line which are sequentially and fixedly arranged on the slide glass; and each antigen strip is formed by separately marking out at least two of antiprotease PR3, antiprotease (MPO) and glomerular basement membrane (GBM) on a nitrocellulose membrane or a nylon membrane. The kit is provided with the ingenious cut-off control strips, one cut-off control strip can interpret two or more detection strips (antigen strips) simultaneously and the judgment result is simpler and more reliable.