The invention relates to an anti-gluing method for a flexible board in a groove region of a rigid-flexible board, comprising the following steps of: firstly, arranging an adhesive tape above a flexible board region which is adjacent to a rigid board region of the rigid-flexible board and is provided with a groove between the rigid board region and the flexible board region; secondly, laminating the rigid-flexible board; and thirdly, removing the adhesive tape. According to the anti-gluing method for the flexible board in the groove region of the rigid-flexible board provided by the invention, the adhesive tape is directly pasted on the flexible board region as a protection layer, the protection layer is removed after the rigid-flexible board is laminated, and excessive glue also can be removed with the protection layer even the excessive glue overflows in a laminating process of the rigid-flexible board, so that the protection layer can ensure that no residual excessive glue of prepreg is left on a cover film used for protecting the flexible board in the groove region, and further the complete appearance of the cover film is guaranteed.