The invention relates to a method for manufacturing a micro-fluidic chip by ultraviolet cured optical cement and belongs to the technical field of micro-fluidic chips. The method comprises the following steps of putting surrounding weirs on a metal coating sputtering substrate, pouring ultraviolet cured optical cement into a pace surrounded by the surrounding weirs, covering a blank film mask, a film mask printed with micro-fluidic chip channel-shaped patterns, and a film mask printed with liquid injection holes on the surrounding weirs, carrying out ultraviolet curing by a photoetching machine to obtain a base layer, a middle layer and a cover plate layer, aligning the base layer, the middle layer and the cover plate layer, and carrying out ultraviolet irradiation bonding by the photoetching machine to obtain the micro-fluidic chip. The method overcomes defects of the traditional mold plate manufacturing technology, is efficient and fast, has simple processes, and can realize manufacture of a space multilayer multidimensional microstructure. The method for manufacturing a micro-fluidic chip is suitable for amplification and separation analysis of a trace amount of DNA fragments having different length values, for separation analysis of amino acids and proteins, and for separation analysis of organic and inorganic small molecules and metal ions.