The invention relates to a mask process of daily necessities, in particular to an application of residual primary culture fluids of mammalian cells in the preparation of active matters of a mask. According to the invention, the residual cell culture fluids are sources of growth factor type matters at home and abroad, and are inexpensive and high in quality, renewable, safe and reliable; silk fibroins can be effectively degraded at the normal temperature by enzymes contained in the residual cell culture fluids, so that small molecular silk fibroins are obtained, and the operation is simple and safe; then sericin in the silk fibroins has immunogenicity so that anaphylaxis may be caused, and the posterior silk gland-derived silk fibroins are completely free of sericin so that the possibility of anaphylaxis is greatly reduced; and the film-forming property of a silk mask is the difficulty of the preparation, a silk I conformation of protein molecules is gradually transformed to silk II in the process of repeatedly stirring and drying the silk fibroins at a high temperature, so that the silk fibroins are stable in structure and have excellent mechanical properties.