The invention discloses a rapid testing method for authentic Chinese rhubarb seeds. The method comprises the steps of 1), obtaining seeds to be tested, grinding, extracting by using methyl alcohol, filtering to obtain filter liquid, volatilizing a solvent, conducting acid hydrolysis and extracting by using an organic solvent to obtain a water layer; and 2), using the water layer obtained in the step 1), adding strong ammonia water of 1-3 times of the volume, shaking, and until no reddish brown or brown flocculent precipitation exist, identifying that the seeds to be tested are authentic Chinese rhubarb seeds. The rapid testing method for authentic Chinese rhubarb seeds is convenient to use, high in accuracy, low in cost and good in market application prospect.