The invention discloses a preparation method of a solid titanium microneedle for cutaneous penetration, comprising the following steps of: processing dies for preparing the solid titanium microneedle; and putting a pre-treated titanium plate among the dies and preparing the solid titanium microneedle through a micro-pier extruding method. The dies are processed through the following concrete steps of: processing a planar male die and a female die with a boss through a line-cutting method and then grinding the female die and the male die, wherein a recess with the size as same as the size of the solid titanium microneedle is arranged on the female die with the boss. Compared with the prior art, the preparation method disclosed by the invention has the advantages that the biocompatibility of the microneedle is improved due to the adoption of pure titanium or titanium alloy, the use reliability of the microneedle is improved through the integration of a needle head and a base, the production efficiency is increased and batch production is favorably realized through die formation.